ANTIBACTERIAL ACTIVITIES OF BIO SYNTHETIC SILVER NANOPARTICLES AGAINST E.COLI ATCC 8739 USING MORINGA OLEIFERA STEM EXTRACT

Abstract

Objectives: Antibacterial activity of bio synthetic silver nanoparticles against E. 
coliATCC8739using Moringa oleifera stem extractwas investigated. 
Materials and methods: Prepared silver nanoparticles were characterized by using different 
techniques included: Ultraviolet Visible Spectroscopy (UV-VIS spectra analysis), Fourier 
Transform Infrared Spectroscopy (FTIR) and Transmission Electron Microscope (TEM). The 
comparison between the antibacterial activities of the concentration 100µg/ml and 80 µg/mlof the
bio synthetic silver nanoparticles using Moringa oleifera stem extract tested against E. 
coliATCC8739was evaluated by using agar diffusion assay method.
Results: FTIR analysis was showed the groups caused the reduction action of Moringa oleifera 
stem extractto form silver nanoparticles. TEM shows a dispersion of nanoparticles in a range (7.56 
- 23.71 - 26.13) nm by using Moringa oleifera stem extract. The antimicrobial activity of the 100 
µg/ml and 80 µg/ml concentrations of synthetized silver nanoparticles was approved againstE.coli 
ATCC8739reference strainby showing zone of inhibition equal to (17.5,16.5)mm respectively, 
while the Moringa oleifera stem extract solution showed no zone of inhibition appeared