CYTOTOXIC ANALYSIS OF SEED EXTRACT OF CYCAS CIRCINALIS L. USING THE ALLIUM CEPA CHROMOSOME ABERRATION ASSAY
Keywords:
Cytotoxic abnormalities, Cycas circinalis, Allium cepa, mito-depressive activity, chromosomal aberrationAbstract
In this study, cytotoxic abnormalities in Allium cepa by the seed extract of Cycas circinalis,
was tested. Different concentrations like 20%, 40%, 60%, 80%, and 100% of seed extract of
Cycas circinalis were prepared after shade drying. In order to compare with standard, a
control was also maintained. The mitotic indices and chromosomal aberration of control and
treatment were calculated. From this study it is proved that, seed extract of Cycas circinalis
has capacity to change mitotic indices and chromosomal make up. From this it is clear that
Cycas circinalis seed extract has mito-depressive activity on cell division. As the
concentration of extract increases, mito-depressive activity also increases. Abnormalities like
Nuclear lesions, Strap shaped nucleus, Chromosome Bridges, Micronuclei and Multinuclei
were also observed in different concentrations.
