INHIBITION OF CRYSTALLIZATION OF CALCIUM OXALATE BYTHE EXTRACTION OF MIMOSA PUDICA (L)

Abstract

Mimosa pudica is used in the treatment ofleprosy, dysentery, vaginal and uterine
complaints, inflammations, burning sensation, asthma, leucoderma, fatigue andblood
diseases.The present study is designed to evaluate the antiurolithiatic activity of aqueous and
ethanolic extracts of different parts of M.pudica. Methods: Thein-vitro inhibition of calcium
oxalate crystal formation byaqueous and ethanolic extracts of leaf, root and flower extracts of M.
pudicaat different concentrations (200, 400, 600, 800 and 1000µg) were investigated by the time
course measurement of turbidity changes due to the crystal nucleation and aggregation in the
synthetic urine at 620nm by means of a spectrophotometer. Results: The aqueous and
ethanolicextracts of different parts of M. pudicaremarkably inhibits the crystal formation in dose
dependent manner.The aqueous extract ofleaf, root and flowers have more antiurolithic activity
as compared to ethanolic extracts. Leaf extrtact of M. pudicashowedmaximum antiurolithic
activity when compared to other parts. Conclusion: Aqueous extract ofleaf, fruit and flower of
M. pudicashowed maximum inhibitory effect on calcium oxalate crytallisation in synthetic urine
than ethanolic extract. Results guide us for the further detailed investigation and development of
new drugs from this medicinal plant for urolithiasis.