ISOLATION METHODS FOR ASPERGILLUS SPECIES OCCURRING ON PLANT SEEDS

Abstract

The aim of the present work is to study the effect of various methods on isolation ofAspergillus species associated with plant seeds. The blotter methods, potato dextrose agar method and water agar methods suggested by International Seed Testing Association (ISTA) were used in isolation of the seed borne Aspergillus species from plant seeds.   The Aspergillus species were isolated by blotter method, potato dextrose agar method and water agar method. Aspergillus species were identified on the basis of morphological characters. It has clearly indicated that different fungi along Aspergillus species were associated with plant seeds under different condition. It is required to identify Aspergillus species and suggestion of promising treatments for fungal biodeterioration and disease management strategy. Further, this investigation helps to control other plant seed diseases effectively. Blotter method proved the best method to detect Aspergillus species in biodeterioration process than any other methods. However, the lowest fungal infestation was noted on water agar method.