IN VITRO SHOOT ORGANOGENESIS OF SNAKE PLANT (Sansevieria trifasciata L.) AS INFLUENCED BY EXPLANT ORIENTATION ANDSUCROSE CONCENTRATION
Keywords:
In vitro shoots, Leaf explants, Orientation, Sucrose, Snake plantAbstract
This experiment was carried out to study the effect of orientations of explants and
concentrations of sucrose on shoot organogenesis of snake plant (Sansevieria trifasciata L.)
under in vitro conditions. Explants were excised from healthy juvenile leaves and sterilized. Subsequently they were kept horizontal and vertical positions in MS medium containing 3%and 5% of sucrose concentrations. The results showed that sucrose at 3% was the most
suitable concentration to produce the high number of shoots and sucrose at 5% was the most
responsive concentration for the shoot elongation in the cultured leaf explants of snake plant. Further, the result exhibited that the most suitable explant orientation for the shoot induction
and proliferation was horizontal placement of leaf explants.
